pmcherry eps8 Search Results


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Addgene inc pmcherry eps8 mouse
(A) Near-TIRF image of a B16F1 cell co-expressing 3x-mCitrine COBL and <t>mCherry-Eps8.</t> Zoom panels highlight intracellular structures with COBL and Eps8 on opposite sides. Numbers to the right of the structures in the first image correspond to the zoom numbers. Scale bar is 5 μm for left image and 1 μm for zoom images. (B) Line scans (n = 15 scans from 8 cells) along linear intracellular actin structures show the distribution of 3x-mCitrine-COBL (green) and mCherry-Eps8 (magenta) from cells co-expressing 3x-mCitrine-COBL and mCherry-Eps8.
Pmcherry Eps8 Mouse, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A) Near-TIRF image of a B16F1 cell co-expressing 3x-mCitrine COBL and mCherry-Eps8. Zoom panels highlight intracellular structures with COBL and Eps8 on opposite sides. Numbers to the right of the structures in the first image correspond to the zoom numbers. Scale bar is 5 μm for left image and 1 μm for zoom images. (B) Line scans (n = 15 scans from 8 cells) along linear intracellular actin structures show the distribution of 3x-mCitrine-COBL (green) and mCherry-Eps8 (magenta) from cells co-expressing 3x-mCitrine-COBL and mCherry-Eps8.

Journal: Cytoskeleton (Hoboken, N.J.)

Article Title: Impact of cordon-bleu expression on actin cytoskeleton architecture and dynamics

doi: 10.1002/cm.21317

Figure Lengend Snippet: (A) Near-TIRF image of a B16F1 cell co-expressing 3x-mCitrine COBL and mCherry-Eps8. Zoom panels highlight intracellular structures with COBL and Eps8 on opposite sides. Numbers to the right of the structures in the first image correspond to the zoom numbers. Scale bar is 5 μm for left image and 1 μm for zoom images. (B) Line scans (n = 15 scans from 8 cells) along linear intracellular actin structures show the distribution of 3x-mCitrine-COBL (green) and mCherry-Eps8 (magenta) from cells co-expressing 3x-mCitrine-COBL and mCherry-Eps8.

Article Snippet: QuickChange site-directed mutagenesis was also used to generate mutations L1153A, L1154A, I1157A and L1166A in the full length EGFP-COBL construct to generate EGFP-COBL-sever*. pmCherry-Eps8 (mouse) was purchased from Addgene (29779, deposited by C. Merrified) along with pmCherry-syndapin-2 (mouse) (27681, deposited by C. Merrifield) [ Taylor et al., 2011 ] as well as EGFP- and mCherry-tagged variants of UtrCH (26737 and 26740, deposited by W. Bement) [ Burkel et al., 2007 ].

Techniques: Expressing